Team:Groningen/Notebook/Wetwork 1July2012


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Test run 3 of the micro-array harvesting setup.
Morning: pre-culture: brought O/N culture to OD = 0.3, growth 1.45 hours to OD = 0.75.
Diluted culture in microarray setup (to OD = 0.095). Growth 1.45 hours in 37 degrees. End OD = 0.95.
Tomorrow start with OD = 0.06. For real Microarray experiment.
Inoculated Bacillus subtilis in LB (from plate), shake at 37 degrees O/N.


1.)O/N culture of B. subtilis in SMM for transformation tomorrow
2.)O/N E. coli with BBa_I742123 in LB+15ug CM for plasmid isolation tomorrow

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