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Team:Groningen/Notebook/Wetwork 1July2012
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| + | Renske/Arjan <br> <br> | ||
| - | Test run 3 of the micro-array harvesting setup. | + | Test run 3 of the micro-array harvesting setup. <br> |
| - | Morning: pre-culture: brought O/N culture to OD = 0.3, growth 1.45 hours to OD = 0.75. | + | Morning: pre-culture: brought O/N culture to OD = 0.3, growth 1.45 hours to OD = 0.75. <br> |
| - | Diluted culture in microarray setup (to OD = 0.095). Growth 1.45 hours in 37 degrees. End OD = 0.95. | + | Diluted culture in microarray setup (to OD = 0.095). Growth 1.45 hours in 37 degrees. End OD = 0.95. <br> |
| - | Tomorrow start with OD = 0.06. For real Microarray experiment. | + | Tomorrow start with OD = 0.06. For real Microarray experiment. <br> |
| - | Inoculated Bacillus subtilis in LB (from plate), shake at 37 degrees O/N. | + | Inoculated Bacillus subtilis in LB (from plate), shake at 37 degrees O/N. <br><br> |
| - | Nisa/Emeraldo | + | Nisa/Emeraldo<br><br> |
| - | 1.)O/N culture of B. subtilis in SMM for transformation tomorrow | + | 1.)O/N culture of B. subtilis in SMM for transformation tomorrow <br> |
| - | 2.)O/N E. coli with BBa_I742123 in LB+15ug CM for plasmid isolation tomorrow | + | 2.)O/N E. coli with BBa_I742123 in LB+15ug CM for plasmid isolation tomorrow <br> |
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| + | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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Revision as of 17:51, 15 September 2012
Renske/Arjan
Test run 3 of the micro-array harvesting setup.
Morning: pre-culture: brought O/N culture to OD = 0.3, growth 1.45 hours to OD = 0.75.
Diluted culture in microarray setup (to OD = 0.095). Growth 1.45 hours in 37 degrees. End OD = 0.95.
Tomorrow start with OD = 0.06. For real Microarray experiment.
Inoculated Bacillus subtilis in LB (from plate), shake at 37 degrees O/N.
Nisa/Emeraldo
1.)O/N culture of B. subtilis in SMM for transformation tomorrow
2.)O/N E. coli with BBa_I742123 in LB+15ug CM for plasmid isolation tomorrow
Back to notebook
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