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Team:Groningen/Notebook/Wetwork 15August2012
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Sub-culturing the transformats colonies on LB agar plates | Sub-culturing the transformats colonies on LB agar plates | ||
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| + | Nisa | ||
| + | Promoters-pigment cloning into pSB1C3. Molarity ratio plasmid:DNA insert = 1:3. | ||
| + | Promoter used in this step: fnr and lacI | ||
| + | Pigment used in this step: lycopene, amilCP, amilGFP | ||
| + | Ligation was done for 2 hours in room temperature before continue to E. coli transformation. | ||
<html> | <html> | ||
Revision as of 13:02, 26 September 2012
Tom
Ligation of alsT(150, 250, 300, 500) with GFP and psb1c3 and pfu promotor with GFP and psb1c3. Result: Transformation in E.coli was succesfull.
PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu. Result: only PCR product: GFP preffix suffix was succesfull.
Emeraldo Sub-culturing the transformats colonies on LB agar plates
Nisa Promoters-pigment cloning into pSB1C3. Molarity ratio plasmid:DNA insert = 1:3. Promoter used in this step: fnr and lacI Pigment used in this step: lycopene, amilCP, amilGFP Ligation was done for 2 hours in room temperature before continue to E. coli transformation.
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