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From 2012.igem.org

E.coli transformed with Vio-biobrick (BBa_K274002)did not grow.

Repeated transformation of E.coli with:

Vio (BBa_K274002)- Kanamycin resistant Red with pBAD promotor (BBa_K274120)- Ampicillin resistant Red without promotor (BBa_K274100) - Ampicillin restistant Red with constitutive promotor (BBa_K274110) - Ampicillin resistant

Standard protocol for E.coli transformation used (see 11th of june).