"
Team:Groningen/Notebook/Wetwork 10August2012
From 2012.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
Result: | Result: | ||
No GFP was seen for all the samples. | No GFP was seen for all the samples. | ||
| + | |||
| + | Renske | ||
| + | |||
| + | Made minimal components medium based upon Jon's modeling data ("Jon's broth") | ||
| + | |||
| + | Glucose: 10 g/L | ||
| + | Potassium: 0.2 g/L | ||
| + | Glutamine: 0.1 g/L | ||
<html> | <html> | ||
Revision as of 12:41, 21 September 2012
Tom
E.Coli with alsT promotor and GFP (colonies: 150-1, 300-1, 300-2, 300-3, 300-4) were grown o/n in m9 medium, illuted to OD=0.1 and grown for 2.5 hours. Samples were prepared of OD=0.05 and divided in 96-well plate. GFP fluorescence was measured o/n.
Result: No GFP was seen for all the samples.
Renske
Made minimal components medium based upon Jon's modeling data ("Jon's broth")
Glucose: 10 g/L Potassium: 0.2 g/L Glutamine: 0.1 g/L
Our sponsors:
