Team:Groningen/Data page

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<li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818000" target=_blank>BBa_K818000</a> - <b>Integration vector</b>. Integrates into the chromosome of <i>Bacillus subtilis</i> at the SacA locus and replicates in <i>Escherichia coli</i>: Derived from vector <i>psac-cm</i> (<a href=http://www.sciencedirect.com/science/article/pii/S0147619X04000332 target =_blank>Middleton et al., 2004</a>).<br><br></li>
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<li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818000" target=_blank>BBa_K818000</a> - <b>Integration vector</b>: integrates into the chromosome of <i>Bacillus subtilis</i> at the <i>SacA</i> locus and replicates in <i>Escherichia coli</i>; derived from vector psac-cm (<a href=http://www.sciencedirect.com/science/article/pii/S0147619X04000332 target =_blank>Middleton et al., 2004</a>).<br><br></li>
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<li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818600" target=_blank>BBa_K818600</a> <b>Rotting meat sensor system</b>: Contains the native <i>Bacillus subtilis</i> promoter PsboA (BBa_K818100) and yellow chromoprotein amilGFP (BBa_K592010) with RBS (B0034). PsboA is strongly activated by volatiles from rotten meat, when Bacillus subtilis grows exponentially. Produces a visible yellow color in six hours.<br><br></li>
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<li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818600" target=_blank>BBa_K818600</a> <b>Rotting meat sensor system</b>: contains the native <i>Bacillus subtilis</i> promoter P<i>sboA</i> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a>) and yellow chromoprotein amilGFP (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592010" target=_blank>BBa_K592010</a>) with RBS (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034" target=_blank>BBa_B0034</a>); P<i>sboA</i> is strongly activated by volatiles from rotten meat when <i>B. subtilis</i> grows exponentially. Produces a visible yellow color in six hours.<br><br></li>
</ol>
</ol>
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<br>
<p><b>Data For Pre-existing Parts</b></p>
<p><b>Data For Pre-existing Parts</b></p>
<ol>
<ol>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592010" target=_blank>BBa_K592010</a> <b>Yellow chromoprotein amilGFP </b> (Uppsala 2011): Stable, non-toxic pigment, well perceptible in the visible and fluorescent spectrum. We characterized the working of the part in <i>Bacillus subtilis</i> for the first time.<br><br></li>
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<li><a href="http://partsregistry.org/Part:BBa_K592010:Experience" target=_blank>BBa_K592010</a> <b>Yellow chromoprotein amilGFP </b> (<a href="https://2011.igem.org/Team:Uppsala-Sweden" target=_blank>Uppsala 2011</a>): stable, non-toxic pigment, well perceptible in the visible and fluorescent spectrums. We are the first to characterize the working of the part in <i>Bacillus subtilis</i>.<br><br></li>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592009" target=_blank>BBa_K592009</a> <b>Blue chromoprotein amilCP </b> (Uppsala 2011): Pigment is perceptible in the visible spectrum. We characterized visualization of the part in <i>Bacillus subtilis</i> for the first time. <br><br></li>
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<li><a href="http://partsregistry.org/Part:BBa_K592009:Experience" target=_blank>BBa_K592009</a> <b>Blue chromoprotein amilCP </b> (<a href="https://2011.igem.org/Team:Uppsala-Sweden" target=_blank>Uppsala 2011</a>): pigment  visible by eye. We are the first to characterize the working of the part in <i>Bacillus subtilis</i>.<br><br></li>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_823023" target=_blank>BBa_823023</a> <b>Integration vector</b> (Munich 2012): Integrates into the <i>Bacillus subtilis</i> chromosome at the AmyE locus and replicates in E. coli. We confirmed that it works.<br><br></li>
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<li><a href="http://partsregistry.org/Part:BBa_K823023:Experience" target=_blank>BBa_K823023</a> <b>Integration vector</b> (<a href="https://2012.igem.org/Team:LMU-Munich" target=_blank>LMU Munich 2012</a>): integrates into the <i>Bacillus subtilis</i> chromosome at the <i>AmyE</i> locus and replicates in <i>E. coli</i>. We confirmed that it works.<br><br></li>
</ol>
</ol>
<br>
<br>
<p><b>We've Also Characterized the Following Parts</b></p>
<p><b>We've Also Characterized the Following Parts</b></p>
<ol>
<ol>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a> <b>Promoter PsboA, rotting meat sensor</b>: Identified by transcriptome analysis of <i>Bacillus subtilis</i> subjected to volatiles from spoiled meat. PsboA natively activates production of the antibiotic compound biosubtilosin. It’s weakly constitutive expressed at exponential growth, but is strongly upregulated when subjected to volatiles from spoiled meat.<br><br></li>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a> <b>Promoter P<i>sboA</i>, rotting meat sensor</b>: natively activates production of the antibiotic compound biosubtilosin; weakly constitutive expressed at exponential growth, strongly upregulated when subjected to volatiles from spoiled meat.  Identified by transcriptome analysis of <i>B. subtilis</i> subjected to volatiles from spoiled meat.<br><br></li>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818400" target=_blank>BBa_K818400</a> <b>Promoter PsboA (BBa_K818100) connected to chromoprotein amilCP (BBa_K592009) with RBS (B0034)</b>: PsboA is strongly activated at the presence of volatiles from rotten meat when <i>Bacillus subtilis</i> is in exponential growth. A visible blue/ purple color can be obtained when using this BBa_K818400 inside our <a href=https://2012.igem.org/Team:Groningen/Sticker target =_blank>sticker system</a>.<br><br></li>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818400" target=_blank>BBa_K818400</a> <b>Rotting meat sensor system</b>: promoter P<i>sboA</i>  (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a>) and blue chromoprotein amilCP (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592009" target=_blank>BBa_K592009</a>) with RBS (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034" target=_blank>BBa_B0034</a>). Visible blue/purple color is obtained when using our <a href=https://2012.igem.org/Team:Groningen/Sticker target =_blank>sticker system</a>.<br><br></li>
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</ol>
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Latest revision as of 03:13, 27 October 2012





Data page

Data For Our Favorite New Parts

  1. BBa_K818000 - Integration vector: integrates into the chromosome of Bacillus subtilis at the SacA locus and replicates in Escherichia coli; derived from vector psac-cm (Middleton et al., 2004).

  2. BBa_K818600 Rotting meat sensor system: contains the native Bacillus subtilis promoter PsboA (BBa_K818100) and yellow chromoprotein amilGFP (BBa_K592010) with RBS (BBa_B0034); PsboA is strongly activated by volatiles from rotten meat when B. subtilis grows exponentially. Produces a visible yellow color in six hours.


Data For Pre-existing Parts

  1. BBa_K592010 Yellow chromoprotein amilGFP (Uppsala 2011): stable, non-toxic pigment, well perceptible in the visible and fluorescent spectrums. We are the first to characterize the working of the part in Bacillus subtilis.

  2. BBa_K592009 Blue chromoprotein amilCP (Uppsala 2011): pigment visible by eye. We are the first to characterize the working of the part in Bacillus subtilis.

  3. BBa_K823023 Integration vector (LMU Munich 2012): integrates into the Bacillus subtilis chromosome at the AmyE locus and replicates in E. coli. We confirmed that it works.


We've Also Characterized the Following Parts

  1. BBa_K818100 Promoter PsboA, rotting meat sensor: natively activates production of the antibiotic compound biosubtilosin; weakly constitutive expressed at exponential growth, strongly upregulated when subjected to volatiles from spoiled meat. Identified by transcriptome analysis of B. subtilis subjected to volatiles from spoiled meat.

  2. BBa_K818400 Rotting meat sensor system: promoter PsboA (BBa_K818100) and blue chromoprotein amilCP (BBa_K592009) with RBS (BBa_B0034). Visible blue/purple color is obtained when using our sticker system.






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