Team:Groningen/Construct

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Construct

The standard construct is a combination of biobricks.

Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.

Groningen2012 JP 20120618 pRe-CII-Lycopene Construct A.png

Or, using BBa_K274002 (Reporter) - Violet pigment producer

Groningen2012 JP 20120618 pRe-CII-Violacein Construct A.png

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