Team:Groningen/Construct

From 2012.igem.org

(Difference between revisions)
Line 516: Line 516:
<br>
<br>
<br>
<br>
-
<br>
+
 
-
<br>
+
-
<br>
+
<li>Or, using BBa_K274002 (Reporter) - Violet pigment producer.</li>  
<li>Or, using BBa_K274002 (Reporter) - Violet pigment producer.</li>  
</ol></FONT>
</ol></FONT>
Line 530: Line 528:
</html>
</html>
<html>
<html>
-
<div style="position:absolute; left: 150px; top: 500px; z-index:1;">
+
<div style="position:absolute; left: 200px; top: 500px; z-index:1;">
<img src="https://static.igem.org/mediawiki/2012/1/1c/Groningen2012_JP_20120618_pRe-CII-Lycopene_Construct_A.png" width="640" >
<img src="https://static.igem.org/mediawiki/2012/1/1c/Groningen2012_JP_20120618_pRe-CII-Lycopene_Construct_A.png" width="640" >
</div>
</div>
-
<div style="position:absolute; left: 150px; top: 750px; z-index:1;">
+
<div style="position:absolute; left: 200px; top: 750px; z-index:1;">
<img src="https://static.igem.org/mediawiki/2012/7/7e/Groningen2012_JP_20120618_pRe-CII-Violacein_Construct_A.png" width="640" >
<img src="https://static.igem.org/mediawiki/2012/7/7e/Groningen2012_JP_20120618_pRe-CII-Violacein_Construct_A.png" width="640" >
</div>
</div>

Revision as of 21:26, 17 September 2012




Construct

sticker


germination


volatiles


identification


pigment


construct


death



The standard construct is a combination of biobricks.

  1. Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  2. BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  3. BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.










  4. Or, using BBa_K274002 (Reporter) - Violet pigment producer.


Our sponsors:


Construct

The standard construct is a combination of biobricks.

  • Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  • BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  • BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.
Groningen2012 JP 20120618 pRe-CII-Lycopene Construct A.png
  • Or, using BBa_K274002 (Reporter) - Violet pigment producer
Groningen2012 JP 20120618 pRe-CII-Violacein Construct A.png
Retrieved from "http://2012.igem.org/Team:Groningen/Construct"