Team:Groningen/Construct

From 2012.igem.org

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<font color=#FF6700><b>The Food Warden</b></font> text..
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<FONT COLOR=#ff6700><b>The standard construct is a combination of biobricks.</b><FONT>
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<FONT COLOR=#ffffff SIZE=3pt style="margin-right=100px; margin-left=100px;"><ol>
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<li>Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.</li>  
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<li>BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.</li>
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<li>BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.</li>
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<li>Or, using BBa_K274002 (Reporter) - Violet pigment producer.</li>
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Revision as of 21:24, 17 September 2012




Construct









The standard construct is a combination of biobricks.

  1. Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  2. BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  3. BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.








  4. Or, using BBa_K274002 (Reporter) - Violet pigment producer.


Our sponsors:


Construct

The standard construct is a combination of biobricks.

  • Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
  • BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
  • BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.
Groningen2012 JP 20120618 pRe-CII-Lycopene Construct A.png
  • Or, using BBa_K274002 (Reporter) - Violet pigment producer
Groningen2012 JP 20120618 pRe-CII-Violacein Construct A.png