Team:Groningen/Notebook/Wetwork 28August2012

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Tom/Arjan

Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac.

Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation.

5 overnight cultures of each previous transformation, 26August2012, were incubated.

Result: All the colonies contained empty Psac plasmid.

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