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Team:University College London/LabBook/Week5
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Contents |
Monday (9.7.12)
Aim: Transformation of Key BioBricks
Method
(LOGO) Transformation Protocol 2
Step 1 – Thawing Cells: Use W3100 cell line created in Week 2 (Expt 2.1) Step 3 – Addition of BioBrick: To each 2ml eppendorf, add 1ul of the following BioBricks. Include an extra tube as a control, with no BioBrick added.
| Function | Module | ||
|---|---|---|---|
| BioBrick | BBa_K123003 | Oestrogen Receptor | Detection |
| BBa_K123002 | Oestrogen Response Element | Detection | |
| BBa_K398108 | Salt Tolerance Cluster | Salt Tolerance | |
| BBa_J23100 | Constitutive Promoter | ||
| BBa_J23107 | Constitutive Promoter | ||
| BBa_R0040 | TetR Repressible Promoter | Buoyancy | |
| BBa_J23106 | Constitutive Promoter | ||
| BBa_B0034 | Ribosome Binding Site (RBS) | All | |
| Control | (No BioBricks) | ||
Step 9 – Plating samples on Agar Plates: The table below indicates the chosen inoculation volume (two for each BioBrick) and the correct gel antibiotic concentration for all samples.
4.1
5.3
5.2
