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Team:University College London/LabBook/Week16
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16-1
16-2
Wednesday (26.09.12)
Aim – We would like to determine if our curli producing BBa_K729018 is capable of producing shear resistant biofilms.
1) Cells are grown overnight in 10ml of LB media at 37˚C, 200rpm.
- a) BBa_K729006
- b) W3110 Control
2) Shake flasks are inoculated with 5 ml of cells into LB media (100ml)
- a) Shake flasks contain pre-sterilised polycarbonate discs
3) Shake flasks are run at 37˚C, 200rpm, for 7 hours.
4) Shake flasks are run at 30˚C, 50rpm, for 14 hours.
5) PC discs are removed from flasks, dried, and stained with crystal violet.
