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From 2012.igem.org

BBa_K592010
This BioBrick was intended to be expressed inside E. coli as described by iGEM Uppsala-Sweden 2011. In our project, we successfully expressed it inside Bacillus subtilis

This is the list of incorrect BioBricks that we used from 2012 distribution kit.


BBa_K090403

Name:	Gram-positive Shuttle Vector for Chromosomal Integration
Intended Purpose:	Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol:	Restriction analysis (dual cut with EcoRI and PstI)
Results:	The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is not a single band and shorter than the expected size.
Gel Pic

BBa_I742123

Name:	Multi-host vector pTG262 converted to BioBrick vector
Intended Purpose:	Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol:	Restriction analysis (single cut) and restriction analysis (dual cut)
Results:	The restriction enzymes cut at illegal site(s)
Gel Pic

Remarks

After discovering that we did not receive these BioBrick as described in the registry, we took initiative to make our own Bacillus subtilis backbone to accommodate our project's needs. These unexpected BioBricks cost us some time to reconstruct our backbone plan, but in the end we were able to come up with a new backbone plan, the BBa_K818000.