BBF RFC#:

The minimum information for a qualified BioBrick

1. Purpose

  Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this standardization will reduce the time to locate and find a BioBrick. So it is essential to create a default template of storing the information of BioBricks.

2. Relation to other BBF RFCs

  This BBF RFC is intended to replace the following standards:
  BBF RFC52: Documentation of BioBrick parts

3. Copyright Notice

  Copyright (C) The BioBricks Foundation (2012). All Rights Reserved.

4. Minimum information for a BioBrick

  To build a qualified BioBrick, the following information MUST be included:
      -       Name of the BioBrick
      -       Fundamental type of the BioBrick
      -       General description of the BioBrick
      -       Status of the BioBrick
      -       Categories that the BioBrick falls in
      -       Author and contact information
      -       Sequence and sequence length
      -       Creation date

    4.1 Name of the BioBrick

  A BioBrick MUST contain a name given by the iGEM committee. A name example: BBa_I14050.

    4.2 Fundamental type of the BioBrick

  The author of a BioBrick MUST assign the BioBrick a corresponding type. The choice has been limited by promoter, RBS, coding sequence, terminator, plasmid backbone and others. The choice ‘others’ means that the BioBrick is not a promoter, not a RBS, not coding sequence, not a terminator nor a plasmid backbone. The author can give the detail description following 4.3.
  There can be only one type specified.

    4.3 General description of the BioBrick

  A description is a MUST requirement for a qualified BioBrick. The function of the BioBrick SHOULD be written into the description if available. And characteristics as well as the physical properties and the best experiment environment are RECOMMENDED. Remember that any offended words or anything irrelevant to synthetic biology SHALL NOT be allowed.

    4.4 Status of the BioBrick

  In order to let users clearly know whether the BioBrick is working or not the status of the BioBrick SHOULD be specified. And the choices of the status are recommended as the following: available, building, cancelled, deleted, missing, planning, sent, unavailable.

    4.5 Categories that the BioBrick falls in

  Any BioBrick SHALL fall in at least one category in the following four categories below. The classification MUST be specified to the second level. The form of the BioBrick category SHALL be like this:
// Chassis/ Yeast;
// Function/ Functions/Biofuels
The “//” indicates the beginning of a classification. A Biobrick can have more than one classification if there is any. Here is the classification tree of the BioBricks. There are three levels; different levels are distinguished according to indentation.
  Function
        Biofuels
        Biosynthesis
        Celldeath
        Cellsignalling
        Coliroid
        Conjugation
        Degradation
        Immunology
        Mismatch repair
        Motility
        Odor
        Recombination
        Regulation
        Reporter
        Sensor
        Structure
        Tunorkillingbacteria
        Others
  Chassis
        E.coli
        Yeast
        T7
        Bacillus subtilis
        Others
  Type
        Plasmidbackbone
        Promoter
        RBS
        Terminator
        Protein Coding Sequences
                Biosynthesis
                Chromatin remodeling
                Enzyme
                Ligand
                Lysis
                Membrane
                Receptor
                Selection marker
                Transcriptional regulator
        Others
  Device
        Generator
        Intermediate
        Inverter
        Measurement
        Reporter
        Signalling
                Reciever
                Sender
        Others

    4.6 Author and contact information

  Every BioBrick MUST contain the names of the authors or designers. The contact information, for example the email address, is OPTIONAL. And the project information CAN be written into this part is the author(s) want(s).

    4.7 Sequence and sequence length

  A BioBrick MUST has a specified sequence of base pairs (or single chain). And the sequence MUST be available. Although users can get the sequence length by counting base pairs, convenience will be improved is a sequence length is provided.

    4.8 Creation date

  A BioBrick SHOULD contains a creation date that recorded the date when the BioBrick is created. This is a fundamental information for a BioBrick but not essential.

5. Author’s Contact Information

  Mubing Zhou, mubingzhou@gmail.com
  Junqiu Zhang, melanie19950103@hotmail.com
  Jiankui He, jiankuihe@gmail.com

6. Reference

  Arend Slomp and David Ekkers “BBF RFC 52: Documentation of BioBrick parts” Conboy et al. "Definitions and Measures of Performance for Standard Biological Parts" https://dspace.mit.edu/handle/1721.1/31335

  To see the PDF version of this RFC, please click here!!!!!