The standard construct is a combination of biobricks.
Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.
Or, using BBa_K274002 (Reporter) - Violet pigment producer.
Our sponsors:
Construct
The standard construct is a combination of biobricks.
Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.
BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.
BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.
Or, using BBa_K274002 (Reporter) - Violet pigment producer
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