Team:UNAM Genomics Mexico/Notebook/Protocols
From 2012.igem.org
Protocols
E. Coli Protocols |
|
Bacillus Protocols |
E. coli Protocols
PCR PROTOCOL (50µl)
Buffer Pfu 5 µl
Upper primers 2.5 µl
Lower primers 2.5 µl
MgCL2 1 µl
DNA (depends on concentration, usually 0.5µl)
Enzime Taq Polymerase 1 µl
DNTP’s 8 µl
Water 29.5 µl
Cycles :)
95ºC 4 min
95ºC 1 min
55ºC :30 secs
70ºC 1 min
goto 2:4 30 times
72ºC 5 min