Team:UNAM Genomics Mexico/Notebook/Protocols

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UNAM-Genomics_Mexico


Protocols


E. Coli Protocols

Contents

Bacillus Protocols

E. coli Protocols


PCR PROTOCOL (50µl)


Buffer Pfu 5 µl
Upper primers 2.5 µl
Lower primers 2.5 µl
MgCL2 1 µl
DNA (depends on concentration, usually 0.5µl)
Enzime Taq Polymerase 1 µl
DNTP’s 8 µl
Water 29.5 µl

Cycles :)
95ºC 4 min
95ºC 1 min
55ºC :30 secs
70ºC 1 min
goto 2:4 30 times
72ºC 5 min