Team:Groningen/Notebook/Wetwork 18July2012

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Tom

Incubating Bacillus in LB medium for overnight growth. Next day: growth curves in SMM medium.


Nisa

Possible promoter (inducible promoter) 1. Strong pBAD promoter (BBa_K206000) 2. LacZ regulated lambda pl hybrid promoter (BBa_R0011)

Allows for strong promotion that can be: a) repressed by lacI b) induced by IPTG in E.coli DH5a over a >600 fold range


Pactivator: pRE (BBa_K116603) or pluxR (BBa_R0062) actvator: CII or luxI ... and CIII cd for promoter leakiness

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