Metal AND

Our system consists of a hybrid promoter which contains binding sites for two transcription factors that act as repressors, CzrA and ArsR. In the presence of Met1, CzrA losses affinity for its binding site.

Similarly, if there is Met2 in the cell, ArsR also dissociates itself from its binding site. Taking into account these facts, unless Met1 and Met2 are present in the culture, we can say that the genes under this hybrid promoter will be repressed, just like a AND gate should function!

Repressors

(Tabla de verdad de TFS) https://2012.igem.org/File:UGM_tv_mtf.png

Inputs

(Tabla verdad inputs) https://2012.igem.org/File:UGM_tv_mi.png