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Team:Groningen/Notebook/Wetwork 27June2012
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Tom
Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.
Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
MicroArray experiment: preparations
Arjan/Renske
Made setup for the micro-array experiment: 37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.
