Team:Groningen/Notebook/Wetwork 15August2012
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Tom
Ligation of alsT(150, 250, 300, 500) with GFP and psb1c3 and pfu promotor with GFP and psb1c3. Result: Transformation in E.coli was succesfull.
PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu. Result: only PCR product: GFP preffix suffix was succesfull.
Emeraldo Sub-culturing the transformats colonies on LB agar plates