Team:Goettingen/week2-3

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#3 Chemoreceptor Library - 2nd Week

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V05_08


Amplification of tar gene
  • Experiment:
    PCR was performed over night with the Tar forward and reverse primers using Pfu polymerase and the isolated genomic DNA from DH10B as template according to protocol.


V05_10


Amplification of tar gene
  • Experiment:
    PCR was controlled by running a 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
  • Observations & Results:
    A band at about 1.6 kb was detected. Amplification of tar was successful.


V05_11


Amplification of tar gene
  • Experiment:
    PCR clean-up using peqGOLD Gel Extraction Kit according to the manual.


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