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From 2012.igem.org

Failed BioBricks BBa_K090403

Name Gram-positive Shuttle Vector for Chromosomal Integration

Intended Purpose Plasmid backbone for cloning in Bacillus subtilis

Testing Protocol Restriction analysis (single cut)

Results The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.

BBa_I742123

Name multi-host vector pTG262 converted to BioBrick vector

Intended Purpose plasmid backbone for cloning in Bacilus subtilis

Testing Protocol restriction analysis (single cut) and restriction analysis (dual cut)

Results the restriction enzymes cut at illegal site(s)