Team:TMU-Tokyo/Parts
From 2012.igem.org
Parts
■BBa_K749025
pfrm-frmR-E0040-B0015-J23100-B0034-fdh-B0015
■BBa_K749015
J23100-B0034-fdh4A-fdh4B-B0015
■BBa_K749021
pfrm-frmR-E0040-B0015
Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde (Device1) and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida (Device2).
BBa_K749025, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.
Formate dehydrogenase device. BBa_K749015 produces formate dehydrogenase 4 from Methylobacterium extorquens consecutively (Device3).
Complete formaldehyde detoxification is realized by only BBa_K749025 and BBa_K749015.
Upstream of BBa_K749025, formaldehyde sensor. we use BBa_K749021 in assay for frmR inhibitory.