Team:TMU-Tokyo/Parts

From 2012.igem.org

 


Parts


 
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■Favorite parts introduction


・BBa_K749025


pfrm-frmR-E0040-B0015-J23100-B0034-fdh-B0015





・BBa_K749015


J23100-B0034-fdh4A-fdh4B-B0015




・BBa_K749021


pfrm-frmR-E0040-B0015




Other parts are described in Parts Registry.




Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde using frmR from Escherichia coli (Device1) and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida (Device2).
BBa_K749025, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.


Formate dehydrogenase device. BBa_K749015 produces formate dehydrogenase 4 from Methylobacterium extorquens consecutively (Device3).
Complete formaldehyde detoxification is realized by only BBa_K749025 and BBa_K749015.

Upstream of BBa_K749025, formaldehyde sensor. we use BBa_K749021 in assay for frmR inhibitory.