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From 2012.igem.org

List of failed BioBricks

BBa_K090403

Name:	Gram-positive Shuttle Vector for Chromosomal Integration
Intended Purpose:	Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol:	Restriction analysis (dual cut with EcoRI and PstI)
Results:	The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is not a single band and shorter than the expected size.
Gel Pic

BBa_I742123

Name:	Multi-host vector pTG262 converted to BioBrick vector
Intended Purpose:	Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol:	Restriction analysis (single cut) and restriction analysis (dual cut)
Results:	The restriction enzymes cut at illegal site(s)