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From 2012.igem.org

Tom

Ligation of Fnr promotor + GFP in psb1c3. Ratio of ligation 1:1:1 and 2:1:1 Fnr:GFP:psb1c3.

Result: Transformation in E.coli did not work.

Nisa

alsT promoter PCR -->OK! Proceeding to ligation and transformation tomorrow.


Emeraldo

Another terminator+biobrick suffix and prefix PCR, cut the purified PCR product and pSac-Cm with EcoRI and HindIII, ligate both cut product and then transform E. coli with the ligated product.

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