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From 2012.igem.org

Failed BioBricks BBa_K090403 '''Name''' Gram-positive Shuttle Vector for Chromosomal Integration '''Intended Purpose''' Plasmid backbone for cloning in ''Bacillus subtilis'' '''Testing Protocol''' Restriction analysis (single cut) '''Results''' The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size. BBa_I742123 '''Name''' multi-host vector pTG262 converted to BioBrick vector '''Intended Purpose''' plasmid backbone for cloning in ''Bacilus subtilis'' '''Testing Protocol''' restriction analysis (single cut) and restriction analysis (dual cut) '''Results''' the restriction enzymes cut at illegal site(s)

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