Team:Groningen/Notebook/Wetwork 31August2012
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Tomvanlente (Talk | contribs) (Created page with "{{HeaderGroningen2012}} Tom/Arjan Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI....") |
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Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI. Plasmid with promotor will be cut with EcoRi and SpeI. | Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI. Plasmid with promotor will be cut with EcoRi and SpeI. | ||
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+ | Plated out E.coli with LMU Munich biobricks showed white and purple/reddish colonies. |
Revision as of 08:14, 31 August 2012
Tom/Arjan
Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI. Plasmid with promotor will be cut with EcoRi and SpeI.
Elbrich
Plated out E.coli with LMU Munich biobricks showed white and purple/reddish colonies.