"
Team:University College London/Module 3/Characterisation
From 2012.igem.org
Rwilkinson (Talk | contribs) |
Sednanalien (Talk | contribs) (→Characterisation) |
||
| Line 6: | Line 6: | ||
== Characterisation == | == Characterisation == | ||
| - | Plastic degradation is mediated via a laccase protein. As such, we will be using a laccase enzymatic activity assay to determine the production of laccase. Laccase catalyses the oxidation of syringaldazine | + | Plastic degradation is mediated via a laccase protein. As such, we will be using a laccase enzymatic activity assay to determine the production of laccase. Laccase catalyses the oxidation of <span class="footnote" title="Syringaldazine">syringaldazine</span>, a reaction that exhibits an observable OD change at 530nm. A sample of syringaldazine can be used as a blank in a spectrophotometer, against a sample containing syringaldazine and our laccase sample, allowing the rate of oxidation to be measured, and hence the enzymatic activity of laccase. |
In order to determine the effectiveness of laccase in degrading plastic, we will expose strips of various types of plastic to the laccase expressing bacteria, before viewing the strips under a scanning electron microscope. This will allow us to compare the pitting in plastic samples treated with laccase, to the untreated samples, allowing us to determine the extent of plastic degradation. | In order to determine the effectiveness of laccase in degrading plastic, we will expose strips of various types of plastic to the laccase expressing bacteria, before viewing the strips under a scanning electron microscope. This will allow us to compare the pitting in plastic samples treated with laccase, to the untreated samples, allowing us to determine the extent of plastic degradation. | ||
{{:Team:University_College_London/templates/foot}} | {{:Team:University_College_London/templates/foot}} | ||
Revision as of 10:28, 28 August 2012
Module 3: Degradation
Description | Design | Construction | Characterisation | Modelling | Results | Conclusions
Characterisation
Plastic degradation is mediated via a laccase protein. As such, we will be using a laccase enzymatic activity assay to determine the production of laccase. Laccase catalyses the oxidation of syringaldazine, a reaction that exhibits an observable OD change at 530nm. A sample of syringaldazine can be used as a blank in a spectrophotometer, against a sample containing syringaldazine and our laccase sample, allowing the rate of oxidation to be measured, and hence the enzymatic activity of laccase.
In order to determine the effectiveness of laccase in degrading plastic, we will expose strips of various types of plastic to the laccase expressing bacteria, before viewing the strips under a scanning electron microscope. This will allow us to compare the pitting in plastic samples treated with laccase, to the untreated samples, allowing us to determine the extent of plastic degradation.
