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Team:Groningen/Notebook/Wetwork 3August2012
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Elbrich: Re-do of transformation with BBa_R0011 from distribution kit 2012 and 2009. | Elbrich: Re-do of transformation with BBa_R0011 from distribution kit 2012 and 2009. | ||
| + | |||
| + | Nisa | ||
| + | |||
| + | Received 6 primer-pairs for pigments and promoter candidates.<br> | ||
| + | Primer list:<br> | ||
| + | 1. Fnr promoter <br> | ||
| + | 2. lacI promoter <br> | ||
| + | 3. Lycopene <br> | ||
| + | 4. Violacein <br> | ||
| + | 5. AmilCP <br> | ||
| + | 6. AmilGFP <br> | ||
| + | <br> | ||
| + | Result from transformation : <br> | ||
| + | - B. subtilis (hopefully) containing pSac-Cm+lycopene grew <br> | ||
| + | - all E. coli transformation showed quite amount of colonies. The E.coli containing pSB1C3+alsT promoter showed pink-white colonies, which white colony implies disruption on the iGEM Groningen 2010 gene, therefore we expected that our alsT promoter+GFP segment was inserted.<br> | ||
Revision as of 16:36, 7 August 2012
Elbrich: Re-do of transformation with BBa_R0011 from distribution kit 2012 and 2009.
Nisa
Received 6 primer-pairs for pigments and promoter candidates.
Primer list:
1. Fnr promoter
2. lacI promoter
3. Lycopene
4. Violacein
5. AmilCP
6. AmilGFP
Result from transformation :
- B. subtilis (hopefully) containing pSac-Cm+lycopene grew
- all E. coli transformation showed quite amount of colonies. The E.coli containing pSB1C3+alsT promoter showed pink-white colonies, which white colony implies disruption on the iGEM Groningen 2010 gene, therefore we expected that our alsT promoter+GFP segment was inserted.
