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From 2012.igem.org

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Revision as of 16:16, 26 October 2012

Data For Our Favorite New Parts

1. BBa_K818000 - Integration vector. Integrates into the chromosome of Bacillus subtilis at the SacA locus and replicates in Escherichia coli. Derived from vector psac-cm (Middleton et al., 2004).
   
   
2. BBa_K818600 Rotting meat sensor system: Contains the native Bacillus subtilis promoter PsboA (BBa_K818100) and yellow chromoprotein amilGFP (BBa_K592010) with RBS (BBa_B0034). PsboA is strongly activated by volatiles from rotten meat, when Bacillus subtilis grows exponentially. Produces a visible yellow color in six hours.
   
   

Data For Pre-existing Parts

1. BBa_K592010 Yellow chromoprotein amilGFP (Uppsala 2011): Stable, non-toxic pigment, well perceptible in the visible and fluorescent spectrum. We characterized the working of the part in Bacillus subtilis for the first time.
   
   
2. BBa_K592009 Blue chromoprotein amilCP (Uppsala 2011): Pigment is visible by eye. We characterized visualization of the part in Bacillus subtilis for the first time.
   
   
3. BBa_823023 Integration vector (LMU Munich 2012): Integrates into the Bacillus subtilis chromosome at the AmyE locus and replicates in E. coli. We confirmed that it works.
   
   

We've Also Characterized the Following Parts

1. BBa_K818100 Promoter PsboA, rotting meat sensor: Identified by transcriptome analysis of Bacillus subtilis subjected to volatiles from spoiled meat. PsboA natively activates production of the antibiotic compound biosubtilosin. It’s weakly constitutive expressed at exponential growth, but strongly upregulated when subjected to volatiles from spoiled meat.
   
   
2. BBa_K818400 Rotting meat sensor system: promoter PsboA (BBa_K818100) and blue chromoprotein amilCP (BBa_K592009) with RBS (BBa_B0034). PsboA is strongly activated at the presence of volatiles from rotten meat when Bacillus subtilis is in exponential growth. A visible blue/purple color can be obtained when using our sticker system.