"
Team:Groningen/Notebook/Wetwork 27June2012
From 2012.igem.org
Emeraldo88 (Talk | contribs) |
Emeraldo88 (Talk | contribs) |
||
| Line 15: | Line 15: | ||
[[File:Groningen_RR_20120628_microarraysetup.jpg|thumb|400px|left|Micro-Array Setup]] | [[File:Groningen_RR_20120628_microarraysetup.jpg|thumb|400px|left|Micro-Array Setup]] | ||
| + | |||
Emeraldo/Nisa | Emeraldo/Nisa | ||
Revision as of 17:05, 8 July 2012
Tom
Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.
Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
MicroArray experiment: preparations
Arjan/Renske
Made setup for the micro-array experiment: 37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.
Emeraldo/Nisa
1.) Order SpeI and EcoRI FastDigest Fermentas 2.) Order Plamid High Pure purification kit Roche 3.) B. subtilis transformation with BBa_K090403 4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length. 5.) Digestion of BBa_K090403. Result: incorrect length.
