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Team:UNAM Genomics Mexico/prueba
From 2012.igem.org
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13.AmyE 5’ ArsR-CzrA 98 E/S. <br /> | 13.AmyE 5’ ArsR-CzrA 98 E/S. <br /> | ||
15.AmyE 5’ ArsR-CzrA 99 E/S. <br /> | 15.AmyE 5’ ArsR-CzrA 99 E/S. <br /> | ||
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| + | <img src='https://static.igem.org/mediawiki/2012/5/55/UnamgenomicsoANDcadmio09_14_2012_5.jpg' height="300"/> | ||
| + | <div class='captionverde'> | ||
| + | <p class='captionInside'>1.ArsR-CzrA 97 E/P. <br /> | ||
| + | 2.ArsR-CzRA 98 E/P. <br /> | ||
| + | 3.1 kb ladder. <br /> | ||
| + | 4.ArsR-CzRA 99 E/P. <br /> | ||
| + | 5.CI 02 E/P. <br /> | ||
| + | 6.AmyE 5’ 97 E/S. <br /> | ||
| + | 7.AmyE 5’ 98 E/S. <br /> | ||
| + | 8.1 kb ladder. <br /> | ||
| + | 9.AmyE 5’ 99 E/S. <br /> | ||
| + | 10.LasR PCR. <br /> | ||
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</li> | </li> | ||
Revision as of 23:20, 18 October 2012
= MAY =
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1.ArsR-CzrA 97 E/P.
2.ArsR-CzrA 98 E/P.
3.ArsR-CzrA 99 E/P.
4.CI 02 E/P.
5.P4 PCR.
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We extracted the correct band by kit
1.ArsR-CzrA 97 E/P.
2.ArsR-CzrA 98 E/P.
3.ArsR-CzrA 99 E/P.
4.CI 02 E/P.
5.P4 PCR.
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1. 1 kb ladder.
2.00179 Lasr lysis
. 3.00179 LasR.
4.00179 Lasr (-).
5.LasR 00179 new primers.
6.LasR 00179 new primers.
7.LasR 00179 new primers (-).
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1.1 kb ladder.
3.ArsR-CzrA 97 E/P.
5.ArsR-CzrA 98 E/P.
7.ArsR-CzrA 99 E/P.
9.CI 02 E/P.
11.AmyE 5’ ArsR-CzrA 97 E/S.
13.AmyE 5’ ArsR-CzrA 98 E/S.
15.AmyE 5’ ArsR-CzrA 99 E/S.
-
1.ArsR-CzrA 97 E/P.
2.ArsR-CzRA 98 E/P.
3.1 kb ladder.
4.ArsR-CzRA 99 E/P.
5.CI 02 E/P.
6.AmyE 5’ 97 E/S.
7.AmyE 5’ 98 E/S.
8.1 kb ladder.
9.AmyE 5’ 99 E/S.
10.LasR PCR.
05/29/2012
Our group is in charge of building part of the “and” construction. We started analyzing if the plasmid we have with P4 actually had what we needed.
The plasmid PRMn25 contains the protein P4. It has Amp100 resistance and comes in Escherichia coli NFI. Cells were lysed to make sure the plasmid was present in these cells (5000bp). We ran a gel with 3 lysis, a sample from the plasmid PFRC54 (A3 promoter) and a sample of total DNA from the strain from which PFRC54 was obtained.
