"
Team:TMU-Tokyo/Notebook/Assay 1 Protocol and Result
From 2012.igem.org
| Line 31: | Line 31: | ||
<Br> | <Br> | ||
<B>■Assay</B><Br> | <B>■Assay</B><Br> | ||
| - | <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/ | + | <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_0_Protocol_and_Result">Assay0</A><Br> |
Device1 Assay</A><Br> | Device1 Assay</A><Br> | ||
<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_2_Protocol_and_Result">Device2 Assay<Br> | <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_2_Protocol_and_Result">Device2 Assay<Br> | ||
Latest revision as of 02:26, 27 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay
Assay 1
■Assay1 Protocol
1. In LB medium (liquid), wound bacteria frm-GFP, were incubated for 6 hours at room shaking culture.
2. Was placed 5μl (0.04%) formalin solution to take the sample tube 500μl culture was diluted 200-fold there.
3.4 Degrees, I was centrifuged for 2 minutes at 120rpm
4. Supernatant was removed, I was vortexed
5. Put on the preparations taking 3μl from the tube, I observed a cover glass.
6. I observed as a control those that do not contain formaldehyde.
■Assay1 Result
GFP expression was not observed either.
50 times, 100 times, 400 times, 600 times, 800 times, created in 1000-fold diluted solution of the same procedure, but I was the same experiment, the expression of GFP was not observed either.
