Team:University College London/LabBook/Week16

(Difference between revisions)

Revision as of 02:06, 27 September 2012

Aim – We would like to determine if our curli producing BBa_K729018 is capable of producing shear resistant biofilms.

1) Cells are grown overnight in 10ml of LB media at 37˚C, 200rpm.

a) BBa_K729006

b) W3110 Control

2) Shake flasks are inoculated with 5 ml of cells into LB media (100ml)

a) Shake flasks contain pre-sterilised polycarbonate discs

3) Shake flasks are run at 37˚C, 200rpm, for 7 hours.

4) Shake flasks are run at 30˚C, 50rpm, for 14 hours.

5) PC discs are removed from flasks, dried, and stained with crystal violet.

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-==16-3==
+== Wednesday (26.09.12) ==
+'''Aim''' – We would like to determine if our curli producing BBa_K729018 is capable of producing shear resistant biofilms.
+) Cells are grown overnight in 10ml of LB media at 37˚C, 200rpm.
+:a) BBa_K729006
+:b) W3110 Control
+) Shake flasks are inoculated with 5 ml of cells into LB media (100ml)
+:a) Shake flasks contain pre-sterilised polycarbonate discs
+) Shake flasks are run at 37˚C, 200rpm, for 7 hours.
+) Shake flasks are run at 30˚C, 50rpm, for 14 hours.
+) PC discs are removed from flasks, dried, and stained with crystal violet.
 <html>
 </div><div class="experiment"></div></html>