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	Tom		Tom
-	Inoculation of E. coli containing LuxI and LuxR.	+	Plasmid isolation of LuxR, LuxI and backbone (BBa_090403) biobricks.
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		+	Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
	'''MicroArray experiment: preparations'''		'''MicroArray experiment: preparations'''

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Revision as of 09:56, 2 July 2012

Tom

Plasmid isolation of LuxR, LuxI and backbone (BBa_090403) biobricks.

Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.

MicroArray experiment: preparations

Arjan/Renske

Made setup for the micro-array experiment: 37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.