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Team:Groningen/Notebook/Wetwork 31August2012
From 2012.igem.org
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Prepared digests for the construction of psac_cmt+pLacI+amilCP and psac_cmt+pLacI+amilGFP again for future use.<br> | Prepared digests for the construction of psac_cmt+pLacI+amilCP and psac_cmt+pLacI+amilGFP again for future use.<br> | ||
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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Latest revision as of 18:55, 26 September 2012
Tom/Arjan
Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI. Plasmid with promotor will be cut with EcoRi and SpeI.
Elbrich
Plated out E.coli with LMU Munich biobricks showed white and purple/reddish colonies.
Yonathan
Prepared digests for the construction of psac_cmt+pLacI+amilCP and psac_cmt+pLacI+amilGFP again for future use.
Back to notebook
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