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Team:TMU-Tokyo/Notebook/Assay
From 2012.igem.org
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<p class="description"> | <p class="description"> | ||
<b>■Assay3(Device3)</b><Br> | <b>■Assay3(Device3)</b><Br> | ||
| - | + | ・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)<Br> | |
| + | ・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ | ||
at 340nm.<Br> | at 340nm.<Br> | ||
→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result">Assay 3 Protocol and Result</A><Br><Br> | →<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result">Assay 3 Protocol and Result</A><Br><Br> | ||
Revision as of 15:12, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Assay
■Assay0
Resistant formaldehyde and formate→Assay 0 Protocol and Result
■Assay1(Device1)
Detect formaldehyde →Assay 1 Protocol and Result
■Assay2(Device2)
Resistant formaldehyde and Quantitative Analysis of Formaldehyde by Formaldehyde Test wako
→Assay 2 Protocol and Result
■Assay3(Device3)
・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)
・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺
at 340nm.
→Assay 3 Protocol and Result
