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Team:TMU-Tokyo/Notebook/Assay 1 Protocol and Result
From 2012.igem.org
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<b>■Assay1 Protocol</b><Br> | <b>■Assay1 Protocol</b><Br> | ||
| - | <Br><Br> | + | <Br> |
| + | 1. In LB medium (liquid), wound bacteria frm-GFP, were incubated for 6 hours at room shaking culture.<Br> | ||
| + | 2. Was placed 5μl (0.04%) formalin solution to take the sample tube 500μl culture was diluted 200-fold there.<Br> | ||
| + | 3.4 Degrees, I was centrifuged for 2 minutes at 120rpm<Br> | ||
| + | 4. Supernatant was removed, I was vortexed<Br> | ||
| + | 5. Put on the preparations taking 3μl from the tube, I observed a cover glass.<Br> | ||
| + | 6. I observed as a control those that do not contain formaldehyde.<Br> | ||
| + | <Br> | ||
Revision as of 15:01, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Assay 1
■Assay1 Protocol
1. In LB medium (liquid), wound bacteria frm-GFP, were incubated for 6 hours at room shaking culture.
2. Was placed 5μl (0.04%) formalin solution to take the sample tube 500μl culture was diluted 200-fold there.
3.4 Degrees, I was centrifuged for 2 minutes at 120rpm
4. Supernatant was removed, I was vortexed
5. Put on the preparations taking 3μl from the tube, I observed a cover glass.
6. I observed as a control those that do not contain formaldehyde.
■Assay1 Result
