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Team:Groningen/Notebook/Wetwork 9August2012
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We found one tiny red E. coli colony (hopefully) containing pSB1C3-fnr promoter-lycopene. This colony will be checked further with PCR (Fnr forward primer-lycopene reverse primer) after plasmid isolation. <br> | We found one tiny red E. coli colony (hopefully) containing pSB1C3-fnr promoter-lycopene. This colony will be checked further with PCR (Fnr forward primer-lycopene reverse primer) after plasmid isolation. <br> | ||
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| - | The other transformation showed pink-white E. coli selection, where pink colony implies pSB1C3-gene insert from iGEM Groningen 2010 and white colony implies the disrupted iGEM Groningen 2010's gene and hopefully it was reverted to our gene construct. Based on this knowledge we took 4 white colonies from each transformation plate, subculture it, and the plasmid isolation will be executed on the next day. | + | The other transformation showed pink-white E. coli selection, where pink colony implies pSB1C3-gene insert from iGEM Groningen 2010 and white colony implies the disrupted iGEM Groningen 2010's gene and hopefully it was reverted to our gene construct. Based on this knowledge we took 4 white colonies from each transformation plate, subculture it, and the plasmid isolation will be executed on the next day.<br> |
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[[File:Groningen2012_AP_20120809_Ecoli pSB1C3-Fnr promoter-lycopene-zoomed.jpg|400px|thumb|left|The small red colony probably contains a construct of fnr promoter-rbs-lycopene.]] | [[File:Groningen2012_AP_20120809_Ecoli pSB1C3-Fnr promoter-lycopene-zoomed.jpg|400px|thumb|left|The small red colony probably contains a construct of fnr promoter-rbs-lycopene.]] | ||
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Revision as of 13:26, 26 September 2012
We found one tiny red E. coli colony (hopefully) containing pSB1C3-fnr promoter-lycopene. This colony will be checked further with PCR (Fnr forward primer-lycopene reverse primer) after plasmid isolation.
The other transformation showed pink-white E. coli selection, where pink colony implies pSB1C3-gene insert from iGEM Groningen 2010 and white colony implies the disrupted iGEM Groningen 2010's gene and hopefully it was reverted to our gene construct. Based on this knowledge we took 4 white colonies from each transformation plate, subculture it, and the plasmid isolation will be executed on the next day.
[[File:Groningen2012_AP_20120809_Ecoli pSB1C3-Fnr promoter-lycopene-zoomed.jpg|400px|thumb|left|The small red colony probably contains a construct of fnr promoter-rbs-lycopene.]]
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