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Team:TMU-Tokyo/Notebook/Assay 1 Protocol and Result
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<p class="description"> | <p class="description"> | ||
<b>■Assay1 Protocol</b><Br> | <b>■Assay1 Protocol</b><Br> | ||
| - | + | <Br><Br> | |
| + | I. Assay of resistance for formaldehyde<Br> | ||
| + | 1. Make mediums<Br> | ||
| + | Agar medium's recipe<Br> | ||
| + | Agar mediums<Br> | ||
| + | Dissolve parl core agar medium 37.5 g into DW 200 ml.<Br> | ||
| + | Autoclave for 20 minutes at 121 °C.<Br> | ||
| + | <Br> | ||
| + | Solution mediums<Br> | ||
| + | Dissolve glucose 2 g, polypeptone 2 g and yeast extract 2 into DW 200 ml.<Br> | ||
| + | Autoclave for 20 minutes at 121 °C.<Br> | ||
| + | <Br> | ||
| + | Phosphate buffer<Br> | ||
| + | Make disodium hydrogen-phosphate aq 300 ml (0.2 M) and sodium<Br> | ||
| + | dihydrogenphosphate dihydrate aq 300 ml (0.2 M) for pH 6.8.<Br> | ||
| + | <Br> | ||
| + | 2. Cultivate E. coli in solution mediums for overnight.<Br> | ||
| + | 3. Prepare 1ml 0.1 %, 1 %, 5 % and 10 % solution of formaldehyde and formate.<Br> | ||
| + | 4. Add 1 μl E. coli into these solutions for 10 minutes.<Br> | ||
| + | 5. Plate on an agar medium.<Br> | ||
| + | 6. Incubate at 37 °C for overnight.<Br> | ||
| + | <Br> | ||
| + | II. Additional experiment for formaldehyde<Br> | ||
| + | 1. Prepare 1 ml 0.04 %, 0.08 %, 0.12 %, 0.16 % and 0.2 % of | ||
| + | formaldehyde solution.<Br> | ||
| + | 2. Add 1 μl E. coli into these solutions for 10 minutes.<Br> | ||
| + | 3. Plate on an agar medium.<Br> | ||
| + | 4. Incubate at 37 °C for overnight.<Br> | ||
| + | |||
| + | |||
| + | </p> | ||
<p class="description"> | <p class="description"> | ||
<b>■Assay1 Result</b><Br> | <b>■Assay1 Result</b><Br> | ||
Revision as of 12:30, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Assay 1
■Assay1 Protocol
I. Assay of resistance for formaldehyde
1. Make mediums
Agar medium's recipe
Agar mediums
Dissolve parl core agar medium 37.5 g into DW 200 ml.
Autoclave for 20 minutes at 121 °C.
Solution mediums
Dissolve glucose 2 g, polypeptone 2 g and yeast extract 2 into DW 200 ml.
Autoclave for 20 minutes at 121 °C.
Phosphate buffer
Make disodium hydrogen-phosphate aq 300 ml (0.2 M) and sodium
dihydrogenphosphate dihydrate aq 300 ml (0.2 M) for pH 6.8.
2. Cultivate E. coli in solution mediums for overnight.
3. Prepare 1ml 0.1 %, 1 %, 5 % and 10 % solution of formaldehyde and formate.
4. Add 1 μl E. coli into these solutions for 10 minutes.
5. Plate on an agar medium.
6. Incubate at 37 °C for overnight.
II. Additional experiment for formaldehyde
1. Prepare 1 ml 0.04 %, 0.08 %, 0.12 %, 0.16 % and 0.2 % of
formaldehyde solution.
2. Add 1 μl E. coli into these solutions for 10 minutes.
3. Plate on an agar medium.
4. Incubate at 37 °C for overnight.
■Assay1 Result
