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Team:Groningen/Notebook/Wetwork 27June2012
From 2012.igem.org
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with rotting/fresh meat. <br> | with rotting/fresh meat. <br> | ||
Filters and peristaltic pump inbetween. <br> | Filters and peristaltic pump inbetween. <br> | ||
| - | Culture is stirred by a magnetic stirrer.<br> | + | Culture is stirred by a magnetic stirrer.<br><br> |
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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Latest revision as of 16:52, 25 September 2012
Tom
Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.
Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
Emeraldo/Nisa
1.) Order SpeI and EcoRI FastDigest Fermentas
2.) Order Plamid High Pure purification kit Roche
3.) B. subtilis transformation with BBa_K090403
4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
5.) Digestion of BBa_K090403. Result: incorrect length.
MicroArray experiment: preparations
Arjan/Renske
Made setup for the micro-array experiment:
37 degrees room. Flask with 50 mL culture
of Bacillus subtilis sp. 168,
connected with tubes to 100 mL flask
with rotting/fresh meat.
Filters and peristaltic pump inbetween.
Culture is stirred by a magnetic stirrer.
Back to notebook
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