Team:Goettingen/week1-1

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<h2><b>V05_02 </b></h2><br>
<h2><b>V05_02 </b></h2><br>
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<b>V05_02</b><br>
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<b>V05_02: Motility of <i>E. coli</i> on different agar concentrations</b><br>
<ul>
<ul>
<li>Experiment: <br>Agar-media (1% LB) with different Agar concentrations (0.150% to 0.975%, in 0.075% steps) were used. The Δtar-strain were used.</li>
<li>Experiment: <br>Agar-media (1% LB) with different Agar concentrations (0.150% to 0.975%, in 0.075% steps) were used. The Δtar-strain were used.</li>
<li>Observations & Results: <br> >0.45% agar: no migration is visible; 0.375% agar: ~3 mm corona is visible. Only little movement; 0.3% agar: strong movement. About 7 mm corona is visible; >0.225% agar: bacteria in whole medium. Obviously too little agar concentrations; 0.3% agar: bright  corona (2.5 cm) is visible; 0.45% agar: now a corona is visible. A side colony runs faster than the rest of the corona. Potential speed mutation.</li>
<li>Observations & Results: <br> >0.45% agar: no migration is visible; 0.375% agar: ~3 mm corona is visible. Only little movement; 0.3% agar: strong movement. About 7 mm corona is visible; >0.225% agar: bacteria in whole medium. Obviously too little agar concentrations; 0.3% agar: bright  corona (2.5 cm) is visible; 0.45% agar: now a corona is visible. A side colony runs faster than the rest of the corona. Potential speed mutation.</li>
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<h2><b>V05_04 </b></h2><br>
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<b>V05_04_1: <i>E. coli</i> movement-dependence of EDTA concentrations</b><br>
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<ul>
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<li>Experiment: <br>Beef(0.3 %)-Trypton-agar with the concentrations 0.5 % and 1.0 % Trypton were made with and without 10^-6 M EDTA.</li>
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<li>Observations & Results: <br> All colonies show a small halo. Only the experiment without EDTA and 1% Trypton shows a big corona. The EDTA only got a negative influence to the swarming.</li>
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</ul>
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<br>
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<b>V05_04_2: Comparison of movement-speed of Δtar strain and potential speed-mutation (see V05_02)</b><br>
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<ul>
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<li>Experiment: <br> 0.3 % agar LB-medium were made. Original ΔTar strain and possible speed mutant were tested.</li>
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<li>Observations & Results: <br> The original ΔTar strain shows a bigger corona as the potential speed mutant. </li>
</ul>
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Latest revision as of 15:18, 25 September 2012

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#1 Selection / Swimming - 1st Week

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V05_02


V05_02: Motility of E. coli on different agar concentrations
  • Experiment:
    Agar-media (1% LB) with different Agar concentrations (0.150% to 0.975%, in 0.075% steps) were used. The Δtar-strain were used.
  • Observations & Results:
    >0.45% agar: no migration is visible; 0.375% agar: ~3 mm corona is visible. Only little movement; 0.3% agar: strong movement. About 7 mm corona is visible; >0.225% agar: bacteria in whole medium. Obviously too little agar concentrations; 0.3% agar: bright corona (2.5 cm) is visible; 0.45% agar: now a corona is visible. A side colony runs faster than the rest of the corona. Potential speed mutation.

V05_04


V05_04_1: E. coli movement-dependence of EDTA concentrations
  • Experiment:
    Beef(0.3 %)-Trypton-agar with the concentrations 0.5 % and 1.0 % Trypton were made with and without 10^-6 M EDTA.
  • Observations & Results:
    All colonies show a small halo. Only the experiment without EDTA and 1% Trypton shows a big corona. The EDTA only got a negative influence to the swarming.

V05_04_2: Comparison of movement-speed of Δtar strain and potential speed-mutation (see V05_02)
  • Experiment:
    0.3 % agar LB-medium were made. Original ΔTar strain and possible speed mutant were tested.
  • Observations & Results:
    The original ΔTar strain shows a bigger corona as the potential speed mutant.


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