"
Team:Groningen/Notebook/Wetwork 22August2012
From 2012.igem.org
(Difference between revisions)
Emeraldo88 (Talk | contribs) |
|||
| Line 4: | Line 4: | ||
<div style="margin-left: 100px; margin-right:100px; color:white; font-size:12pt;"> | <div style="margin-left: 100px; margin-right:100px; color:white; font-size:12pt;"> | ||
| - | Yonathan and Elbrich | + | Yonathan and Elbrich<br> |
Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm. | Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm. | ||
| - | Violacein had not the right size, so ligation of the construct was not possible. | + | Violacein had not the right size, so ligation of the construct was not possible.<br> |
| + | |||
| + | Emeraldo<br> | ||
| + | Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator with ligation reaction, E. coli DH5alpha transformation with the ligated product. | ||
<html> | <html> | ||
Revision as of 15:14, 25 September 2012
Yonathan and Elbrich
Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.
Violacein had not the right size, so ligation of the construct was not possible.
Emeraldo
Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator with ligation reaction, E. coli DH5alpha transformation with the ligated product.
Our sponsors:
