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Team:Bordeaux/Tenth
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Revision as of 11:03, 24 September 2012
Day 44 : 03-09-2012
Today we did a miniprep of the precultures and runned a gel to test the plasmids.
Day 45 : 06-09-2012
Today we did the ligations for IVA5AIVB , 5AIVDIVEIVC and 5AIIIC then inactivated the ligase after 1h 20 °C incubation and put overnight at 4°C
Day 46 : 07-09-2012
We transformed the ligations from yesterday plus biobricks IA ,IB ,IC ,IE and two new bricks IA' and IB' to make a new stock We then put the plate at room temperature over the weekend
