Team:METU/Safety

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<html lang="en-US" xmlns="http://www.w3.org/1999/xhtml" dir="ltr">
-
!align="center"|[[Team:METU|Home]]
+
    <head>
-
!align="center"|[[Team:METU/Team|Team]]
+
        <meta http-equiv="Content-type" content="text/html; charset=utf-8" />
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=METU Official Team Profile]
+
-
!align="center"|[[Team:METU/Project|Project]]
+
-
!align="center"|[[Team:METU/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:METU/Modeling|Modeling]]
+
-
!align="center"|[[Team:METU/Notebook|Notebook]]
+
-
!align="center"|[[Team:METU/Safety|Safety]]
+
-
!align="center"|[[Team:METU/Attributions|Attributions]]
+
-
|}
+
 +
        <link rel="shortcut icon" href="http://simsekburak.com/hasan/css/images/favicon.ico" />
 +
        <link rel="stylesheet" href="http://simsekburak.com/hasan/css/style.css" type="text/css" media="all" />
 +
        <!--[if IE 6]>
 +
                <link rel="stylesheet" href="css/ie6.css" type="text/css" media="all" />
 +
                <script src="http://simsekburak.com/hasan/js/png-fix.js" type="text/javascript"></script>
 +
        <![endif]-->
 +
        <script src="http://simsekburak.com/hasan/js/jquery-1.4.2.js" type="text/javascript"></script>
 +
        <script src="http://simsekburak.com/hasan/js/jquery.jcarousel.js" type="text/javascript"></script>
 +
        <script src="http://simsekburak.com/hasan/js/js-func.js" type="text/javascript"></script>
-
== Safety Questions ==
+
        <style>
 +
            h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{
 +
                display:none;
 +
            }
 +
            #top-section{
 +
                height: 20px !important;
 +
                border: none !important;
 +
            }
-
'''1.Would any of your project ideas raise safety issues in terms of:'''
+
            div#content{
 +
                padding: 0 !important;
 +
                background: transparent;
 +
                border: none !important;
 +
                width: auto;
 +
            }
-
'''• researcher safety,'''
+
        </style>
-
- There are no hazardous chemicals that we used in the laboratory except for EtBr that we used in a special room for gel visualization and some buffers used in commercially available kits.
 
-
'''• public safety, or'''
+
    </head>
 +
    <body>
 +
        <!-- Header -->
 +
        <div id="header">
 +
            <div class="shell">
 +
                <h1 id="logo" class="notext"><a href="https://2012.igem.org/Team:METU/test">
 +
                        <img width="200px" height="104px" src="/wiki/images/2/22/METU_logo.png" style="padding-top: 10px;"></img>
 +
                    </a>
 +
                </h1>
 +
                <h1 id="logo2" class="notext"><a href="https://2012.igem.org/Main_Page">
 +
                        <img width="144px" height="109px" src="/wiki/images/0/08/Igem_logo.png" style="padding-top: 25px;"></img></a></h1>
 +
                <div id="navigation">
 +
                    <ul>
 +
                        <li><a href="https://2012.igem.org/Team:METU/test" class="active"><span>Home</span></a></li>
-
- Since all the organisms used in the wetlab are destroyed after they are done working with they pose no danger for public safety. We use E.coli strain DH5alpha which is easy to kill and all the student members who work in the wetlab are very well trained to make sure there is no unwanted contamination. This year we are also working on a kill switch which, if works properly, will eliminate any bacteria whereas they will not be able to survive without IPTG present. Also we want to clone the final circuit into cyanobacteria which is not as harmful for public as E.coli.
+
                        <li><a href="https://2012.igem.org/Team:METU/test/Team"><span>Team</span></a></li>
 +
                        <li><a href="https://2012.igem.org/Team:METU/test/CODH"><span>CODH</span></a>
 +
                            <div class="dd-holder">
 +
                                <div class="dd-t"></div>
 +
                                <div class="dd">
 +
                                    <ul>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CODH#Overview">Overview</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CODH#Modelling">Modelling</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CODH#Results">Results</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CODH#Biobricks">Biobricks</a></li>
-
'''• environmental safety?'''
+
                                    </ul>
 +
                                    <div class="cl">&nbsp;</div>
 +
                                </div>
 +
                                <div class="dd-b"></div>
 +
                            </div>
 +
                        </li>
 +
                        <li><a href="https://2012.igem.org/Team:METU/test/KillSwitchOverview"><span>Kill-Switch</span></a>
 +
                            <div class="dd-holder">
 +
                                <div class="dd-t"></div>
 +
                                <div class="dd">
 +
                                    <ul>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/KillSwitchOverview#Overview">Overview</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/KillSwitch/Modelling#Modelling">Modelling</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/KillSwitchOverview#Results">Results</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/KillSwitchOverview#Biobricks">Biobricks</a></li>
-
- As mentioned above a kill switch will be available in the organism which will initiate cell death when the cell is in an environment that does not contain enough IPTG.
+
                                    </ul>
 +
                                    <div class="cl">&nbsp;</div>
 +
                                </div>
 +
                                <div class="dd-b"></div>
 +
                            </div>
 +
                        </li>
-
'''2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?'''
+
                        <li><a href="https://2012.igem.org/Team:METU/test/CellLimiter"><span>Cell Limiter</span></a>
 +
                            <div class="dd-holder">
 +
                                <div class="dd-t"></div>
 +
                                <div class="dd">
 +
                                    <ul>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CellLimiter#Overview">Overview</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CellLimiter#Modelling">Modelling</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CellLimiter#Results">Results</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/CellLimiter#Biobricks">Biobricks</a></li>
-
No parts in our new biobrick parts will raise any safety issues. We are even able to increase safety thanks to the kill switch and cell limiter that we have designed.
+
                                    </ul>
 +
                                    <div class="cl">&nbsp;</div>
 +
                                </div>
 +
                                <div class="dd-b"></div>
 +
                            </div>
 +
                        </li>
-
'''3.Is there a local biosafety group, committee, or review board at your institution?'''
+
                        <li><a href="https://2012.igem.org/Team:METU/test/Overview"><span>Notebook</span></a>
 +
                            <div class="dd-holder">
 +
                                <div class="dd-t"></div>
 +
                                <div class="dd">
 +
                                    <ul>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/Overview">Overview</a></li>
 +
                                        <li><a href="/Team:METU/Timeline">Timeline</a></li>
 +
                                        <li><a href="/Team:METU/Brainstorm">Brainstorm</a></li>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/test/Protocols">Protocols</a></li>
 +
                                        <li><a href="/Team:METU/CloningPlan">Cloning Plan</a></li>
 +
                                        <li class="last"><a href="/Team:METU/Judging">Judging</a></li>
 +
                                    </ul>
 +
                                    <div class="cl">&nbsp;</div>
 +
                                </div>
 +
                                <div class="dd-b"></div>
 +
                            </div>
 +
                        </li>
 +
                        <li><a href="#"><span>Human Practice</span></a>
 +
                            <div class="dd-holder">
 +
                                <div class="dd-t"></div>
 +
                                <div class="dd">
 +
                                    <ul>
 +
                                        <li><a href="https://2012.igem.org/Team:METU/Gallery">Gallery</a></li>
 +
                                        <li class="last"><a href="/Team:METU/Activities">Activities</a></li>
 +
                                    </ul>
 +
                                    <div class="cl">&nbsp;</div>
 +
                                </div>
 +
                                <div class="dd-b"></div>
 +
                            </div>
 +
                        </li>
 +
                        <li><a href="#"><span>Extras</span></a>
 +
                            <div class="dd-holder">
 +
                                <div class="dd-t"></div>
 +
                                <div class="dd">
 +
                                    <ul>
 +
                                        <li><a href="/Team:METU/Safety">Safety</a></li>
-
'''• If yes, what does your local biosafety group think about your project?'''
+
                                        <li class="last"><a href="/Team:METU/Attributions">Attributions</a></li>
-
'''• If no, which specific biosafety rules or guidelines do you have to consider in your country?'''
+
                                    </ul>
 +
                                    <div class="cl">&nbsp;</div>
 +
                                </div>
 +
                                <div class="dd-b"></div>
 +
                            </div>
-
In our university we have a biosafety and ethical research center which monitors the safety of our projects. http://www.ueam.metu.edu.tr  The members of METU Biology department are also members of National Biosafety Coordinating Committee. We are able to consult to them whenever it is necessary. Also biosafety is a very important subject for METU Department of Biology, whereas the student members are very well trained in the subject. Also the projects that we decided to work on are presented to many people in the area in order to see if they are of any concern for the biosafety regulations. All the procedures that are used in the lab are also approved procedures that are used by these people mentioned above.
+
                        </li>
 +
                    </ul>
 +
                </div>
 +
                <div class="cl">&nbsp;</div>
 +
                <!-- <div class="search">
 +
                                <form action="#" method="post">
 +
                                        <span class="field">
 +
                                                <input type="text" class="blink" value="Search" title="Search" />
 +
                                        </span>
 +
                                        <input type="submit" class="search-btn notext" value="Submit" />
 +
                                </form>
 +
                        </div> 
 +
                -->
-
'''4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?'''
+
            </div>
 +
        </div>
 +
        <!-- End Header -->
 +
        <!-- Slider -->
 +
        <div id="slider2">
 +
            <div class="shell">
 +
                <div class="slider-holder2">
 +
                    <h1>Safety</h1>
 +
                    <div class="cl">&nbsp;</div>
-
We believe using specific strains designed specifically for labwork will be safer. It is possible to genetically modify the organisms so that, they are not able to grow outside the lab environment. There probably is not a way to build some biobricks that can be modified in order tomake
+
                </div>
 +
            </div>
 +
        </div>
 +
        <!-- End Slider -->
 +
        <!-- Main -->
 +
        <div id="main">
 +
            <div class="shell">
 +
                <p>
 +
                    <font size="5">
 +
                        <b>Safety Questions</b>
 +
                    </font>
 +
                </p>
 +
                <p>
 +
                    <b>1.Would any of your project ideas raise safety issues in terms of:</b>
 +
                </p>
 +
                <ul>
 +
                    <li><b>researcher safety,</b>
 +
                        <ul>
 +
                            <li>There are no hazardous chemicals that we used in the laboratory except for EtBr that we used in a special room for gel visualization and some buffers used in commercially available kits. </li>
 +
                        </ul>
 +
                    </li>
 +
                    <li><b>public safety, or</b>
 +
                        <ul>
 +
                            <li>Since all the organisms used in the wetlab are destroyed after they are done working with they pose no danger for public safety. We use E.coli strain DH5alpha which is easy to kill and all the student members who work in the wetlab are very well trained to make sure there is no unwanted contamination. This year we are also working on a kill switch which, if works properly, will eliminate any bacteria whereas they will not be able to survive without IPTG present. Also we want to clone the final circuit into cyanobacteria which is not as harmful for public as E.coli.</li>
 +
                        </ul>
 +
                    </li>
 +
                    <li><b>environmental safety?</b>
 +
                        <ul>
 +
                            <li>As mentioned above a kill switch will be available in the organism which will initiate cell death when the cell is in an environment that does not contain enough IPTG.</li>
 +
                        </ul>
 +
                    </li>
 +
                </ul>
 +
 
 +
                <p>
 +
                    <b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</b>
 +
                </p>
 +
                <p>No parts in our new biobrick parts will raise any safety issues. We are even able to increase safety thanks to the kill switch and cell limiter that we have designed.</p>
 +
 
 +
                <p>
 +
                    <b>3.Is there a local biosafety group, committee, or review board at your institution?</b>
 +
                </p>
 +
                <ul>
 +
                    <li><b>If yes, what does your local biosafety group think about your project?</b></li>
 +
                    <li><b>If no, which specific biosafety rules or guidelines do you have to consider in your country?</b></li>
 +
                </ul>
 +
 
 +
                <p>
 +
                    In our university we have a biosafety and ethical research center which monitors the safety of our projects. http://www.ueam.metu.edu.tr  The members of METU Biology department are also members of National Biosafety Coordinating Committee. We are able to consult to them whenever it is necessary. Also biosafety is a very important subject for METU Department of Biology, whereas the student members are very well trained in the subject. Also the projects that we decided to work on are presented to many people in the area in order to see if they are of any concern for the biosafety regulations. All the procedures that are used in the lab are also approved procedures that are used by these people mentioned above.
 +
                </p>
 +
 
 +
                <p>
 +
                    <b>4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</b>
 +
                </p>
 +
                <p>
 +
                    We believe using specific strains designed specifically for labwork will be safer. It is possible to genetically modify the organisms so that, they are not able to grow outside the lab environment. There probably is not a way to build some biobricks that can be modified in order to make
 +
                </p>
 +
            </div>
 +
        </div>
 +
 
 +
        <!-- End Main -->
 +
        <!-- Footer -->
 +
        <div id="footer">
 +
            <div class="shell">
 +
                <p class="left">Copyright &copy; 2010 RightDirection. All rights reserved. Designed by <a href="http://chocotemplates.com" target="_blank" title="The Sweetest CSS Templates WorldWide">Chocotemplates.com</a></p>
 +
                <p class="right">
 +
                    <a href="#">Home</a>
 +
                    <span>|</span>
 +
                    <a href="#">About</a>
 +
                    <span>|</span>
 +
                    <a href="#">Team</a>
 +
                    <span>|</span>
 +
                    <a href="#">Jobs</a>
 +
                    <span>|</span>
 +
                    <a href="#">Contact Us</a>
 +
                    <span>|</span>
 +
                    <a href="#">Portfolio</a>
 +
                    <span>|</span>
 +
                    <a href="#">Blog</a>
 +
                </p>
 +
                <div class="cl">&nbsp;</div>
 +
            </div>
 +
        </div>
 +
        <!-- Footer -->
 +
    </body>
 +
</html>

Revision as of 11:05, 23 September 2012

Safety

 

Safety Questions

1.Would any of your project ideas raise safety issues in terms of:

  • researcher safety,
    • There are no hazardous chemicals that we used in the laboratory except for EtBr that we used in a special room for gel visualization and some buffers used in commercially available kits.
  • public safety, or
    • Since all the organisms used in the wetlab are destroyed after they are done working with they pose no danger for public safety. We use E.coli strain DH5alpha which is easy to kill and all the student members who work in the wetlab are very well trained to make sure there is no unwanted contamination. This year we are also working on a kill switch which, if works properly, will eliminate any bacteria whereas they will not be able to survive without IPTG present. Also we want to clone the final circuit into cyanobacteria which is not as harmful for public as E.coli.
  • environmental safety?
    • As mentioned above a kill switch will be available in the organism which will initiate cell death when the cell is in an environment that does not contain enough IPTG.

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?

No parts in our new biobrick parts will raise any safety issues. We are even able to increase safety thanks to the kill switch and cell limiter that we have designed.

3.Is there a local biosafety group, committee, or review board at your institution?

  • If yes, what does your local biosafety group think about your project?
  • If no, which specific biosafety rules or guidelines do you have to consider in your country?

In our university we have a biosafety and ethical research center which monitors the safety of our projects. http://www.ueam.metu.edu.tr The members of METU Biology department are also members of National Biosafety Coordinating Committee. We are able to consult to them whenever it is necessary. Also biosafety is a very important subject for METU Department of Biology, whereas the student members are very well trained in the subject. Also the projects that we decided to work on are presented to many people in the area in order to see if they are of any concern for the biosafety regulations. All the procedures that are used in the lab are also approved procedures that are used by these people mentioned above.

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

We believe using specific strains designed specifically for labwork will be safer. It is possible to genetically modify the organisms so that, they are not able to grow outside the lab environment. There probably is not a way to build some biobricks that can be modified in order to make

Retrieved from "http://2012.igem.org/Team:METU/Safety"