Team:UNAM Genomics Mexico/Notebook/ANDSugar
From 2012.igem.org
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<li> | <li> | ||
<img src='https://static.igem.org/mediawiki/2012/5/56/UnamgenomicsandsugarBitacora_5.jpg' height="300"/> | <img src='https://static.igem.org/mediawiki/2012/5/56/UnamgenomicsandsugarBitacora_5.jpg' height="300"/> | ||
- | <div class=' | + | <div class='captionverde'> |
<p class='captionInside'>1. 1 kb ladder <br /> | <p class='captionInside'>1. 1 kb ladder <br /> | ||
pHp45 Ω with E/P<br /> | pHp45 Ω with E/P<br /> | ||
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> Ran a gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_electrophoresis | GEL ELECTROPHORESIS PROTOCOL]] . (5) <br /> | > Ran a gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_electrophoresis | GEL ELECTROPHORESIS PROTOCOL]] . (5) <br /> | ||
>Extracted from gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_extraction_protocol | LIQUID CULTURE]]. <br /> | >Extracted from gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_extraction_protocol | LIQUID CULTURE]]. <br /> | ||
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ARAC<br /> | ARAC<br /> | ||
>From the overnight plate we prepared liquid culture [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Liquid_culture | LIQUID CULTURE]]. <br /> | >From the overnight plate we prepared liquid culture [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Liquid_culture | LIQUID CULTURE]]. <br /> | ||
>We left them incubating overnight. <br /> | >We left them incubating overnight. <br /> | ||
- | >We left a plate (LB Km | + | >We left a plate (LB Km DH5α C0080 and a control). <br /> |
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+ | <h2>06/18/12</h2><br /> | ||
+ | >Plasmid extraction AraC with kit. <br /> | ||
+ | >C0080-psB2K3 915 bp<br /> | ||
+ | >Streaked 4 LB Km 30 plates DH5α psB2K3. <br /> | ||
+ | >It seemed as though there were two different colonies (white) and there had been a mutation. Normally they are red. <br /> | ||
+ | >Ran gel with Spr/Strr (eppendorf -20ºC) [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_electrophoresis | GEL ELECTROPHORESIS PROTOCOL]]. <br /> | ||
+ | >Made glycerols from one tube of DH5α C0080 Km 50 -> 04. <br /> | ||
+ | >Made glycerols from one tube of DH5α PSB4A5 Amp 100 -> 05 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#GLYCEROL_PROTOCOL | GLYCEROL PROTOCOL]]. <br /> | ||
+ | >Digested C0080 X,S. <br /> | ||
+ | >Digested B0014 E,P [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Digestion_protocol_.2820_.C2.B5l.29 | LIQUID CULTURE]]. <br /> | ||
+ | |||
}} | }} |
Revision as of 05:06, 23 September 2012
Contents[hide] |
Arabinose/Xylose AND Gate
Nanotubes!! | The logic | Random info |
06/07/12
PY BROTH 10g salt/L
PSB2K3 kanamicin
We transformed RFP E1010.
plate 1 18F
plate 2 17E
Stock 50 mg/mL
E.coli 1/1000
TRANSFORMATION PROTOCOL.
We made liquid cultures with colonies LIQUID CULTURE.
We extracted plasmids PLASMID EXTRACTION PROTOCOL.
We ran a gel to check the extraction GEL ELECTROPHORESIS PROTOCOL.
06/12/12
We made glycerols of the bacteria
GLYCEROL PROTOCOL .
We transformed plasmid pHp45 TRANSFORMATION PROTOCOL.
06/13/12
We ran gel and extracted from gel LIQUID CULTURE.
We made liquid cultures LIQUID CULTURE and lysed [LYSIS PROTOCOL].
06/14/12
We extracted from gel LIQUID CULTURE.
We made glycerols of pHp45 Ω GLYCEROL PROTOCOL .
We transformed PSB2K3 Km (Kanamycin) 5C BBa_J04450
PSB4A5 Am (ampicillin) 1I BBa_J04450
AraC BBa_C0080
2012 14L plate 1 pSB2K3 Km+
2011 14L plate 1 pSB2K3 Km+
2012 14L plate 1 pSB2K3 Km+
TRANSFORMATION PROTOCOL.
06/15/12
> Digested pHp45 Ω with E/P LIQUID CULTURE.
> We extracted plasmid with kit.
> Ran a gel GEL ELECTROPHORESIS PROTOCOL . (5)
>Extracted from gel LIQUID CULTURE.
ARAC
>From the overnight plate we prepared liquid culture LIQUID CULTURE.
>We left them incubating overnight.
>We left a plate (LB Km DH5α C0080 and a control).
06/18/12
>Plasmid extraction AraC with kit.
>C0080-psB2K3 915 bp
>Streaked 4 LB Km 30 plates DH5α psB2K3.
>It seemed as though there were two different colonies (white) and there had been a mutation. Normally they are red.
>Ran gel with Spr/Strr (eppendorf -20ºC) GEL ELECTROPHORESIS PROTOCOL.
>Made glycerols from one tube of DH5α C0080 Km 50 -> 04.
>Made glycerols from one tube of DH5α PSB4A5 Amp 100 -> 05 GLYCEROL PROTOCOL.
>Digested C0080 X,S.
>Digested B0014 E,P LIQUID CULTURE.