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Team:UNAM Genomics Mexico/Notebook/ANDSugar
From 2012.igem.org
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| + | <h2>06/12/12</h2><br /> | ||
We made glycerols of the bacteria <br /> [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#GLYCEROL_PROTOCOL | GLYCEROL PROTOCOL]] . <br /> | We made glycerols of the bacteria <br /> [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#GLYCEROL_PROTOCOL | GLYCEROL PROTOCOL]] . <br /> | ||
| - | We transformed plasmid | + | We transformed plasmid pHp45 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Transformation | TRANSFORMATION PROTOCOL]]. <br /> |
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| + | <img src='https://static.igem.org/mediawiki/2012/3/38/UnamgenomicsandsugarBitacora_2.jpg' height="300"/> | ||
| + | <div class='captionnaranja'> | ||
| + | <p class='captionInside'>1. 1 kb ladder <br /> | ||
| + | 2.E1010 | ||
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| + | <h2>06/13/12</h2><br /> | ||
| + | We ran gel and extracted from gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_extraction_protocol | LIQUID CULTURE]].<br /> | ||
| + | We made liquid cultures [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Liquid_culture | LIQUID CULTURE]] and lysed [LYSIS PROTOCOL]. <br /> | ||
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| + | <h2>06/14/12</h2><br /> | ||
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Revision as of 04:44, 23 September 2012
Contents |
Arabinose/Xylose AND Gate
| Nanotubes!! | The logic | Random info |
-
1. 1 kb ladder
2.E1010
06/07/12
PY BROTH 10g salt/L
PSB2K3 kanamicin
We transformed RFP E1010.
plate 1 18F
plate 2 17E
Stock 50 mg/mL
E.coli 1/1000
TRANSFORMATION PROTOCOL.
We made liquid cultures with colonies LIQUID CULTURE.
We extracted plasmids PLASMID EXTRACTION PROTOCOL.
We ran a gel to check the extraction GEL ELECTROPHORESIS PROTOCOL.
06/12/12
We made glycerols of the bacteria
GLYCEROL PROTOCOL .
We transformed plasmid pHp45 TRANSFORMATION PROTOCOL.
-
1. 1 kb ladder
2.E1010
06/13/12
We ran gel and extracted from gel LIQUID CULTURE.
We made liquid cultures LIQUID CULTURE and lysed [LYSIS PROTOCOL].
