Team:Groningen/Construct
From 2012.igem.org
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- | < | + | <FONT COLOR=#ff6700><b>The standard construct is a combination of biobricks.</b><FONT> |
+ | <br> | ||
+ | |||
+ | <FONT COLOR=#ffffff SIZE=3pt style="margin-right=100px; margin-left=100px;"><ol> | ||
+ | <li>Backbone:Available in registry: BBa_K090403 (Backbone) - Single copy shuttle vector. Other option: build our own well-characterized backbone for our chassis.</li> | ||
+ | |||
+ | <li>BBa_K116632+BBa_K116602 (Positive feedback loop) - Uses the pRe promoter regulated by C11 and terminated by B0010. Other options: BBa_J37019;BBa_K116639; BBaK116609.</li> | ||
+ | |||
+ | |||
+ | <li>BBa_K274100 (Reporter) - Modified carotenoid pathway to produce red pigment.</li> | ||
+ | <br> | ||
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+ | <li>Or, using BBa_K274002 (Reporter) - Violet pigment producer.</li> | ||
+ | </ol></FONT> | ||
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+ | <img src="https://static.igem.org/mediawiki/2012/1/1c/Groningen2012_JP_20120618_pRe-CII-Lycopene_Construct_A.png" width="640" > | ||
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+ | <img src="https://static.igem.org/mediawiki/2012/7/7e/Groningen2012_JP_20120618_pRe-CII-Violacein_Construct_A.png" width="640" > | ||
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Revision as of 21:24, 17 September 2012