Team:Groningen/Notebook/Wetwork 28August2012
From 2012.igem.org
(Difference between revisions)
Tomvanlente (Talk | contribs) |
|||
Line 1: | Line 1: | ||
{{HeaderGroningen2012}} | {{HeaderGroningen2012}} | ||
+ | |||
+ | |||
+ | <div style="margin-left: 100px; margin-right:100px; color:white; font-size:12pt;"> | ||
Tom | Tom | ||
Line 12: | Line 15: | ||
Result: All the colonies contained empty Psac plasmid. | Result: All the colonies contained empty Psac plasmid. | ||
+ | |||
+ | <html> | ||
+ | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
+ | </html> | ||
+ | |||
+ | {{Template:SponsorsGroningen2012}} |
Revision as of 20:12, 16 September 2012
Tom
Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac.
Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation.
Tom/Arjan
5 overnight cultures of each previous transformation, 26August2012, were incubated.
Result: All the colonies contained empty Psac plasmid.